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Methods for Preparation H7 Flagellin and Polyclonal Antibodies for Detection of H7 Antigens of E. Con O157:H7
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Abstract: E coli of the serotype 0157:H7 has recently been isolated from
cattle and human fecal samples in Egypt. The aim of this study is to develop an effective method for extraction of flagella from E. coli OI57:H7.
Flagella were prepared through the following steps: dissociated by incubation of bacteria, ethanol treatment, supernatant collection, and finally precipitated by centrifugation. Flagellar filaments in E.coli O157:H7 were demonstrated by transmission electron microscopy and by PCR .H7 flagellin proteins were shown to be immunogenic. They induced a systemic immune response against those H7 proteins when injected SIC into animals or when given orally.
Polyclonal antiserum raised to H7 flagellin proteins reacted with intact E.coli 0157:H7. Also, this antiserum reacted strongly with E.coli 011:K+.H34 and O156:K-:H23. .In effect, the prepared polyclonal sera have the potential to serve as high-quality diagnostic reagent.
Keywords: flagellar antigen; polyclonal H7 antiserum; Transmission Electron Microscopy (TEM) and (EHEC) Entrohemorragic E. coli O157:H7
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| Publication year |
2008
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| Availability location |
المعمل المركزى للنظم الخبيرة
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| Availability number |
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| Organization Name |
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| City |
القاهرة
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| serial title |
مؤتمر الشرق الأوسط وشمال أفريقيا لبحوث الثروة الحيوانية
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| Author(s) from ARC |
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AGROVOC
TERMS |
Antigens.
Polyclonal antibodies.
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| Proposed Agrovoc |
H7;
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| Publication Type |
Conference/Workshop
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