Preparation Two Specific Stained Antigens for the Detection of Escherichia Coli O157:H7 Infection and Bru Cellosis
Abstract: Escherichia coli 015 7 antigen share elements with Brucella smooth strains which cause cross reactivity in serological standard tests for Brucellosis. In infected animals with Escherichia coli O 157:H7 , antibodies against E. coli cross react with the Brucella antigens (Strain 99 Rose Bengal Test). Consequently, Brucellosis is difficult to diagnosis because shared with E. coli 0157:H7. The aim of this study was to elaborate on a method of antigen preparation that is useful in detecting specific antibodies against each bacterial strain without cross reactivity. In this study, sera were examined from animals that are: vaccinated with Brucella vaccines (Rev.1 and S19); natura1ly infected with Escherichia coli 0151:H7 and Brucellosis; and experimentally infected with Escherichia coli 0151:H7, wild field Brucella melitensis, and other E. coli serotypes. All those sera were ex-amined for antibodies against the developed two antigens. Cross reaction disappeared.. E. coli 0157:H7 antibodies failed to react with the modified Brucella antigen and Brucella antibodies failed to react with the colored antigen of E. coli 0151:H7 .In effect, we were able to demon-strate mixed infection and prove the effectively of the developed antigens. This implicitly proves that the developed antigens have a high degree of specificity and sensitivity. They could be a useful tool for clinical diagnosis and epidemiological studies of E. coli 0151:H7 in-fection and Brucellosis in animals with potential use in humans.

Keywords: Bacterial antigens, bacterial cross reaction. Brucellosis, Enterohaemorrhagic Escherichia coli, TEM (Transmission Electron Microscopy)
Publication year 2008
Availability location 6 شارع النور نادى الصيد الدقى ميشيل باخوم سابقا
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City القاهرة
serial title مؤتمر الشرق الأوسط وشمال أفريقيا لبحوث الثروة الحيوانية
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AGROVOC
TERMS		 Antigens. Brucellosis. Escherichia coli.
Publication Type Conference/Workshop
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